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Isopropyl-beta-D-thiogalactopyranoside CAS NO.367-93-1
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- Product Details
Keywords
- Isopropyl-beta-D-thiogalactopyranoside 99%
Quick Details
- ProName: Isopropyl-beta-D-thiogalactopyranoside
- CasNo: 367-93-1
- Molecular Formula: C9H18O5S
- Appearance: White to almost white crystalline powd...
- ProductionCapacity: Metric Ton/Day
- Purity: 98% - 101%
- LimitNum: 0 Metric Ton
Superiority
| Appearance | White to almost white crystalline powder |
|
Purity (By HPLC) |
Min 99%+ |
|
Assay by HPLC |
98% - 101% (On Anhydrous Basis) |
Details
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of thelacoperon and induces expression of β-galactosidase inEscherichia coli.The compound is widely used with X-Gal plates to screen for blue and white colonies. Thelacoperon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
| Product Name | Isopropyl-beta-D-thiogalactopyranoside |
| Synonyms | IPTG;Isopropyl-beta-D-thiogalactoside;IPTG, HEMIDIOXANE ADDUCT;ISOPROPYL-1-THIO-BETA-D-GALACTOPYRANOSIDE;ISOPROPYLTHIO-BETA-GALACTOSIDE;ISOPROPYLTHIO-BETA-THIOGALACTOSIDASE;ISOPROPYLTHIO-B-THIOGALACTOPYRANOSIDE;ISOPROPYL THIOGALACTOSIDE;ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE, DIOXANE FREE;Isopropyl β-D-1 Thiogalactopyranoside;propan-2-yl 1-thio-beta-D-galactopyranoside;1-methylethyl 1-thio-D-galactopyranoside |
| Molecular Formula | C9H18O5S |
| Molecular Weight | 238.3012 |
| InChl | InChI=1/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8- ,9?/m1/s1 |
| CAS Registry Number | 367-93-1 |
| Molecular Structure |
|
| Appearance | White to almost white crystalline powder |
|
Purity (By HPLC) |
Min 99%+ |
|
Assay by HPLC |
98% - 101% (On Anhydrous Basis) |
|
Specific optical rotation |
-31.5 ± 3.0° [a]D20(c=1,H2O) |
| Melting Point | 110-114℃ |
| Boiling Point | 438.4°C at 760 mmHg |
|
pH |
5.0 - 7.0 |
| Dioxane free | Not detected |
| Solubility | Soluble in water, and methanol. |
| Water content (BY KF) | Max 1.0% |
| Storage | Shipped at Ambient Temperature. Store at +2 - 8°C. |
| Description |
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of thelacoperon and induces expression of β-galactosidase inEscherichia coli.The compound is widely used with X-Gal plates to screen for blue and white colonies. Thelacoperon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity. |
| Usage |
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Induces protein synthesis inE. coliwhen transcription is controlled by thelac-promoter. In conjunction with X-gal,q.v.,in detection oflacgene activity during cloning experiments.
IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to
|
| Application | An analogue of allolactose that binds specifically to the repressor protein of thelacoperon. |
